Novel immunocontraceptive targets in mammals: uterine secretions and the conceptus; a marsupial approach
by
Menkhorst EM, Cui S, Selwood L.
Department of Zoology, The University of Melbourne, Parkville, Victoria 3010, Australia. Reproduction. 2008 Oct;136(4):471-80.
ABSTRACT
We report the first immunocontraceptive trial in mammals using a uterine-secreted protein, the marsupial shell coat protein 4 (CP4). The marsupial shell coat, which surrounds the conceptus for 60-80% of gestation, is secreted by the uterine epithelium. Following immunization against glutathione S-transferase (GST)-CP4, the fertility of female common brushtail possums (n=6) was significantly reduced (P=0.000), and this reduction in fertility was positively correlated with the maximum GST-CP4 humoral immune response (P=0.025). Ultrastructural examination of the reproductive tract indicated that the cell-mediated immune response against GST-CP4 targeted the shell coat, the shell-free conceptus and the uterine glandular epithelium, thus preventing normal conceptus development and uterine secretion of shell coat proteins and nutrients. These results show that uterine-secreted proteins are promising immunocontraceptive targets, especially in pest mammal species, e.g. possum, rabbit and horse, that have uterine-secreted additions to embryonic coats, or that have late implantation requiring uterine nutrient provisioning from secretions.
‘I’ll find an answer that’s good enough for them,’ he said to himself, as he slipped the letter into his post-box.{246} THE FERRY-BOAT. THE FERRY-BOAT. "Why, I thought it was on a hill, or something of the sort; I had no reason to think so, of course, but I had formed that picture of it." The Clockwork man shook his head. "We have houses, but they are not full of things like yours are, and we don't live in them. They are simply places where we go when we take ourselves to pieces or overhaul ourselves. They are—" his mouth opened very wide, "the nearest approach to fixed objects that we have, and we regard them as jumping-off places for successive excursions into various dimensions. Streets are of course unnecessary, since the only object of a street is to lead from one place to another, and we do that sort of thing in other ways. Again, our houses are[Pg 146] not placed together in the absurd fashion of yours. They are anywhere and everywhere, and nowhere and nowhen. For instance, I live in the day before yesterday and my friend in the day after to-morrow." This principle is somewhat obscure, and the nature of percussive forces not generally considered—a matter which may be illustrated by considering the action of a simple hand-hammer. Few [103] people, in witnessing the use of a hammer, or in using one themselves, ever think of it as an engine giving out tons of force, concentrating and applying power by functions which, if performed by other mechanism, would involve trains of gearing, levers, or screws; and that such mechanism, if employed instead of a hammer, must lack that important function of applying force in any direction as the will and hands may direct. A simple hand-hammer is in the abstract one of the most intricate of mechanical agents—that is, its action is more difficult to analyse than that of many complex machines involving trains of mechanism; yet our familiarity with hammers causes this fact to be overlooked, and the hammer has even been denied a place among those mechanical contrivances to which there has been applied the name of "mechanical powers." ‘When anyone prefers beauty to virtue, what is this but the real and utter dishonour of the soul? For such a preference implies that the body is more honourable than the soul; and this is false, for there is nothing of earthly birth which is more honourable than the heavenly, and he who thinks otherwise of the soul has no idea how greatly he undervalues this wonderful possession.’47 Monday, 3rd hour That is the truth. We left the caravan far behind. In the gorge with its rosy-pink soil the silence was exquisite, the air had the freshness of a mountain height, and quite inexplicably amid these barren rocks, where there was not a sign of vegetation, there was a scent of honey and almonds. Life went on very much the same at the post when there was only the infantry left in possession. As there was nothing to do at any time, there was nothing the less for that. On the principle that loneliness is greatest in a crowd, Stanton was more isolated now[Pg 183] than Grant had been in the days when there had been no railroad west of Kansas. The railroad was through the southwest now, but it was a hundred miles away. It was unsafe to ride outside the reservation, there was no one for hops, the only excitement was the daily addition to the list of slaughtered settlers. Felipa spent most of her time with the Ellton baby. Miss McLane had been married to Landor's second lieutenant for a year and a half, and they were very happy. But Felipa in the knowledge of the strength of her own love, which gained new might each time that she wrestled with it and threw it back upon the solid ground of duty, found their affection decidedly insipid. Like the majority of marital attachments, it had no especial dignity. It was neither the steadfast friendship she felt for her husband, nor the absolute devotion she would have given Cairness. Si strode over to the group, snatched the bat from Harry's hand, spanked him with it, and started for the others of the group. HoME日本1级:A片
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